Isolation and partial purification of theSaccharomyces cerevisiaecytokinetic apparatus
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چکیده
منابع مشابه
Isolation and Partial Purification of Hepatic Stimulatory Substance.
THE PURPOSE of this study was to purify and characterize hepatic stimulatory substance (HSS), a protein found in the liver that has been reported to stimulate hepatic DNA synthesis and hepatocyte replication and that was previously identified by LaBrecque et al l -3 and Francavilla et al in the rat liver,4,5 as well as by Starzl et al in the canine liver.6,7 HSS has been partially purified by s...
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Polyphenol oxidase from Black mulberrys was extracted and partially purified through (NH4)2SO4 precipitation, dialysis and ion exchange chromatography. P-cresol was the better substrate for cresolase activity with a Km (11.5 mM) and catechol for catecholase activity with Km (6.4 mM) at pH 5. Km for cresolase and catecholase activity of IsoPPOII at pH 6.5 were 12 and 8.5 and for IsoPPOIII at pH ...
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An antibody was raised against a protein isolated from the cytoplasm of mesothelioma cells. It was subsequently used in an immunoperoxidase procedure on formalin fixed, paraffin embedded tissue sections. A representative sample of benign and malignant tumours from all the systems of the human body was examined. All the tumours derived from coelomic surfaces (mesotheliomas of pleura, peritoneum,...
متن کاملPartial Purification and Characterization
An enzyme fraction containing phosphatase activity for phosphorylated eukaryotic peptide initiation factor 2 (eIF-2) has been isolated from rabbit reticulocytes and partially characterized, The enzyme efficiently catalyzes release of phosphate from the small subunit of eIF-2 (eIF-2a) that has been phosphorylated by the hemin-controlled repressor. It is shown to restore activity of this phosphor...
متن کاملIsolation and Purification of Dna
IMPORTANT NOTE: The smallest amount of contamination of DNA preparations by recombinant phages or plasmids can be disastrous. Many person-years have been wasted reisolating previously cloned sequences that contaminated preparations of DNA used to create recombinant DNA libraries and many researchers have been embarrassed to find that the “extra” genes they found on their Southern blots were act...
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ژورنال
عنوان ژورنال: Cell Motility and the Cytoskeleton
سال: 2009
ISSN: 0886-1544,1097-0169
DOI: 10.1002/cm.20412