Isolation and Optimization of Hydrocarbon – Degrading Bacteria

Aims: To isolate and characterize hydrocarbon – degraders in a contaminated soil sample
 Study Design: This experiment was carried out under aseptic conditions. The result confirmed by visible spectrophotometer
 Place Duration of Study: Department Biological Sciences, Microbiology Programme, Clifford University, Ihie Campus, Owerrinta, Abia State, Nigeria, between May 2021 to July 2021
 Methodology: Soil sample with used this from an automobile mechanic workshop. A ten-fold serial dilution made for each sample, after which 1 ml 10-5 dilutions plated using pour plate method incubated at 37 0C 48 hrs. isolates were then grown on different hydrocarbons (crude oil, fuel or spent engine oil) the degradation efficiency spectrophotometer.
 Result: isolation degrading bacteria topsoil subsoil samples workshop located Isiala Ngwa North Local Government Area Junction, State out. Four bacterial species isolated collected, subjected degradation/utilization test it observed that two identified as Bacillus Staphylococcus sp able utilize medium more efficiently than other isolates.
 Conclusion: Hydrocarbon auto North, State. Two four strains (Bacillus sp), have highest potential use (fuel, crude oil condemn sole carbon source. These strains' abilities suggest they could be effective bioremediation polluted locations. combat contamination caused motor mechanics who carelessly dispose old environment, well general contamination.