Isolation and molecular identification of pathogenic cryptococcus neoformans from pigeon dropping and human sputum

This study is designed to isolate and molecular identification of C. neoformans, neoformans pathogenic yeast effect immunocompromised immunocompetent. Methods: collect 50 samples from pigeon dropping fanciers (sputum). The collection time was extended November 2021 February 2022, then culture at SDA, BSA, Cryptococcus Differential agar, esculin Eucalyptus leaves agar media Brain heart infusion with methyldopa, biochemical test including urease confirm by PCR technique sequencing genetic analysis. results showed that 3 swaps taken sputum human included cryptococcus 6 bird neoformans. indicated the virulence normal habitat These indicate there may be a risk for exposure cryptococcal disease due inhalation basidiospores in houses