Isolation and Characterization of Rhizobium from Green Gram (Vigna radiata)

Nitrogen is a crucial component that acts as building blocks for nucleic acids and proteins. It abundantly present in atmosphere of the earth but plants are not able to easily utilize it. Diazotrophic microorganisms such Rhizobium convert N2 make it available pulse crops. In study, total thirty-four isolates were recovered, from twenty-two with gram-negative-rods biochemically characterized. Most negative congo red (CR) absorption, glucose-peptone, lactose, hofer’s alkaline assays. These have PGP (plant growth promoting) properties solubilisation mineral, hormone, hydrogen cyanide (HCN) productions along antagonistic activity. Plant study revealed SBGR25 treatment has highest germination per cent (98.0 %), shoot (10.3 cm) total-height (16.6 cm), dry weight (5.60 g). Hence, suggests strains could be used promising bioinoculants crop.