Isolation and Characterization of Novel Butachlor-Degrading Bacteria from Rice Paddy Soils

Four different bacteria capable of degrading butachlor, as well five syntrophic pairs able to break down were isolated from rice paddy soils in Korea. Genetic and phenotypic analyses conducted better understand their characteristics behavior. All single isolates utilize butachlor a sole carbon energy source. Analysis the 16S rRNA sequence showed that related members genus Rhodococcus new type butachlor-degrading Sphingobium. The chromosomal DNA fingerprinting patterns analyzed using technique called repetitive-sequence-based PCR (REP-PCR). results there two REP-PCR found among four independent bacteria, ten strains produced total eight distinct fingerprints. Through use gas chromatography–mass spectrometry (GC-MS) analysis, it was observed pair breaking various chemical pathways, such 2-chloro-N-(2,6-diethylphenyl) acetamide (CDEPA), 2,6-diethylphenyl isocyanate, 2,6-diethylaniline (DEA), 2-ethylaniline.