Isolation and Characterization of Microsporum gypseum Lysosomes: Role of Lysosomes in Macroconidia Germination
نویسندگان
چکیده
منابع مشابه
Heat-induced macroconidia germination in Microsporum gypseum.
A method for obtaining purified ungerminated macroconidia is described, and a technique for obtaining 85 to 90% germination of macroconidia under normal nutritional conditions is presented.
متن کاملRegulation and self-inhibition of Microsporum gypseum Macroconidia germination.
Germination of Microsporum gypseum macroconidia was accompanied by the release of alkaline protease, calcium ions, and inorganic phosphate into the germination fluid. The rate of germination was greatest during the first 2 hr, decreasing thereafter. This decrease in rate was accompanied by a decrease in protease activity, which was caused by an interaction of the enzyme with the inorganic phosp...
متن کاملRelationship of nuclear segregation and macroconidial germination in Microsporum gypseum.
Microsporum gypseum macroconidia germinated at 37 C possessed from one to eight nuclei per germinated spore compartment. The distribution of nuclei per spore compartment was the result of a random packaging of nuclei from the available nuclear population. Partial inhibition of germination by incubation at 25 C or at 37 C in the presence of 10(-4)m phenyl methyl sulfonyl-fluoride resulted in an ...
متن کاملIsolation of pig thyroid lysosomes. Biochemical and morphological characterization.
Open thyroid follicles were prepared by mechanical disruption of pig thyroid fragments through a metal sieve. This procedure allowed preparation of thyroid-cell material depleted of colloid thyroglobulin. Open thyroid follicles were used to prepared a crude particulate fraction, which contained lysosomes, mitochondria and endoplasmic reticulum. These organelles were subfractionated by isopycnic...
متن کاملIsolation and characterization of early endosomes, late endosomes and terminal lysosomes: their role in protein degradation.
Although endosomal proteolysis has been reported (e.g. for peptide hormones and lysosomal enzymes), lysosomes are believed to be the main site of degradation in the endocytic pathway. We have studied the separate roles of lysosomes and prelysosomal endocytic organelles in the degradation of ovalbumin in J774 cells. The ovalbumin was labelled with 125I-tyramine cellobiose (125I-TC-ova). The labe...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1972
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.110.1.354-362.1972