Isolation and Characterization of Human Factor VIII (Antihemophilic Factor)
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چکیده
منابع مشابه
Isolation and Characterization of Human Factor VIII
Factor VIII (antihemophilic factor) has been purified approximately 500.fold from the cryoprecipitate fraction of human plasma. The isolation procedure involves adsorption of contaminants with Al(OH),, column chromatography on tricalcium citrate-cellulose, precipitation with concanavalin A, and an agarose gel filtration step. The linal product is homogeneous when examined by zone electrophoresi...
متن کاملHeterogeneity of human circulating anticoagulants against antihemophilic factor (factor VIII).
The heterogeneity of human circulating anticoagulants against antihemophilic factor (AHF, factor VIII) observed in seven patients, both with and without classic hemophilia, was investigated by neutralization of their activity with antiserums directed to whole IgG and to lambda and kappa light chains. All seven anticoagulants were immunoglobulins. Six appeared to contain both kinds of light chai...
متن کاملIsolation and characterization of human factor VIII: molecular forms in commercial factor VIII concentrate, cryoprecipitate, and plasma.
Human factor VIII has been isolated from a high purity factor VIII concentrate by immunoaffinity chromatography and HPLC on Mono Q gel. Two fractions of factor VIII were obtained with a specific activity of approximately equal to 7000 units/mg. The major fraction contained eight peptide chains of 200, 180, 160, 150, 135, 130, 115, and 105 kDa plus one doublet chain of 80 kDa. The minor fraction...
متن کاملThe Isolation and Characterization of Bovine Factor VIII
Bovine Factor VIII (antihemophilic factor) has been purified approximately lO,OOO-fold with an over-all yield of 10 to 15%. The purification procedure involves BaS04, kaolin, and bentonite adsorption to remove contaminants, ethanol, polyethylene glycol, and /Y-alanine fractionation, calcium citrate-cellulose chromatography, concanavalin A precipitation, and an Agarose gel filtration step. The f...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1973
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)43824-6