Isolation and characterization of homokaryotic strains from the ligninolytic basidiomycete Ceriporiopsis subvermispora
نویسندگان
چکیده
منابع مشابه
Isolation and characterization of homokaryotic strains from the ligninolytic baqsidiomycete Cerioporiopsis subvermispora
Genetic analyses of the lignin-degrading fungus Ceriporiopsis subvermispora is complicated by a dikaryotic nuclear condition and the absence of spore forms. Previous investigations had identified a family of closely related sequences encoding manganese peroxidase (MnP), but the relationship between genes and allelic variants could not be experimentally established. Addressing this issue, homoka...
متن کاملProperties of laccase isoenzymes produced by the basidiomycete Ceriporiopsis subvermispora.
Laccase is one of the ligninolytic enzymes found in liquid cultures of the fungus Ceriporiopsis subvermispora in defined medium. As an approach to a clarification of the role of laccases during the attack on lignin by the fungus, the enzyme has been characterized further. The levels of this phenol oxidase increase 2-fold in the presence of p-anisidine and are severely affected when addition of ...
متن کاملLignin-degrading peroxidases from the genome of the selective ligninolytic fungus Ceriporiopsis subvermispora*
Background: The first genome of a selective lignin-degrader is available. Results: Its screening shows 26 peroxidase genes, and five were heterologously expressed and catalytic properties investigated. Conclusion: Two new peroxidases oxidize simple and dimeric lignin models and efficiently depolymerize lignin. Significance: Although lignin peroxidase and versatile peroxidase had not been report...
متن کاملEffect of manganese on the secretion of manganese-peroxidase by the basidiomycete Ceriporiopsis subvermispora.
The ligninolytic machinery of the widely used model fungus Ceriporiopsis subvermispora includes the enzymes manganese-peroxidase (MnP) and laccase (Lcs). In this work the effect of Mn(II) on the secretion of MnP was studied. Cultures grown in the absence of Mn(II) showed high levels of mnp transcripts. However, almost no MnP enzyme was detected in the extracellular medium, either by enzymatic a...
متن کاملIsolation and characterization of novel bacterial strains exhibiting ligninolytic potential
BACKGROUND To expand on the range of products which can be obtained from lignocellulosic biomass, the lignin component should be utilized as feedstock for value-added chemicals such as substituted aromatics, instead of being incinerated for heat and energy. Enzymes could provide an effective means for lignin depolymerization into products of interest. In this study, soil bacteria were isolated ...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 2001
ISSN: 0378-1097,1574-6968
DOI: 10.1111/j.1574-6968.2001.tb10656.x