Isolation and characterization of germ line DNA from mouse sperm.
نویسندگان
چکیده
منابع مشابه
isolation and enrichment of mouse female germ line stem cells
objective: the existence of female germ-line stem cells (fgscs) has been the subject of a wide range of recent studies. successful isolation and culture of fgscs could facilitate studies on regenerative medicine and infertility treatments in the near future. our aim in the present study was evaluation of the most commonly used techniques in enrichment of fgscs and in establishment of the best p...
متن کاملIsolation and Enrichment of Mouse Female Germ Line Stem Cells
OBJECTIVE The existence of female germ-line stem cells (FGSCs) has been the subject of a wide range of recent studies. Successful isolation and culture of FGSCs could facilitate studies on regenerative medicine and infertility treatments in the near future. Our aim in the present study was evaluation of the most commonly used techniques in enrichment of FGSCs and in establishment of the best pr...
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Aspartokinase has been isolated from wheat germ and a preliminary survey made of its properties in a partially purified extract. The enzyme has an absolute requirement for ATP and a divalent metal ion. The phosphate donor can be either ATP or GTP, but other nucleotides are ineffective. Both magnesium and manganese will activate the enzyme, whereas calcium shows a trace amount of activity. The e...
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nowadays, production of recombinant proteins in eukaryotes is gaining good deal of attention. transgenic chicken as a eukaryotic system has a high potential for producing recombinant proteins. post-translational changes, especially glycosylation, are characteristic of the eukaryotic proteins. in practice we need to choose a proper expressing host when considering over-expression of a recombinan...
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Objective(s):To culture thein vitro mouse embryonic stem cells (mESCs) and to direct their differentiation to germ-line cells; in present study we used a vector backbone containing the fusion construct Stra8-EGFP to select differentiated ES cells that entered meiosis. Retinoic acid was used to differentiate embryonic stem cells to germ cells. Materials and Methods: A fragment of Stra8 gene pr...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1979
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.76.8.3947