Isolation and characterization of an Fe,-S8 ferredoxin (ferredoxin II) from Clostridium thermoaceticum

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Isolation and characterization of an Fe,-S8 ferredoxin (ferredoxin II) from Clostridium thermoaceticum.

A second ferredoxin protein was isolated from the thermophilic anaerobic bacterium Clostridium thermoaceticum and termed ferredoxin II. This ferredoxin was found to contain 7.9 +/- 0.3 iron atoms and 7.4 +/- 0.4 acid-labile sulfur atoms per mol of protein. Extrusion studies of the iron-sulfur centers showed the presence of two [Fe4-S4] centers per mol of protein and accounted for all of the iro...

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A four-iron, four-sulfide ferredoxin with high thermostability from Clostridium thermoaceticum.

A ferredoxin containing only one [Fe4S4] cluster was purified from Clostridium thermoaceticum. It has a molecular weight of about 7,300, a partial specific volume of 0.67, and an isoelectric point of 3.25. Its absorption spectrum has two maxima at 390 nm (epsilon = 16.8 X 10(3)M-1cm-1) and at 280 nm (epsilon = 24.2 X 10(3)M-1cm-1). The absorption at 390 nm is almost half that of other clostridi...

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Isolation and Partial Characterization of Ferredoxin from Zea mays.

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Nondenaturing procedure for rapid preparation of ferredoxin from Clostridium pasteurianum.

Lovcnberg and Williams (1‘1 have reported that, when a solution of Clostridium pastewianum ferredoxin in 90% ammonium sulfate is treated on a column of DEAE-cellulose, ferredoxin is retained by the column. They showed t,hat, ferredoxin is eluted when the ammoniuni sulfate concentration is lowered to about 35c/, saturation. Thcsc observations suggested the method described in this paper for puri...

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Proton magnetic resonance study of ferredoxin from Clostridium pasteurianum.

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ژورنال

عنوان ژورنال: Journal of Bacteriology

سال: 1982

ISSN: 0021-9193,1098-5530

DOI: 10.1128/jb.151.1.328-333.1982