Ischemic-Postconditioning Improves Myocardial Injury and Fibrosis Following Ischemia/Reperfusion Injury in Diabetic Rats Pretreated with Alpha-Lipoic Acid

Background: Myocardial ischemia/reperfusion (I/R) injury is one of the pre-eminent causes death. Prevention I/R in diabetic patients full ups and downs. This study has investigated effect co-administration alpha-lipoic acid (ALA) preconditioning ischemic-postconditioning (Post) on myocardial fibrosis type-II rats suffering from injury. Methods: Chronic diabetes was induced by a high-fat supplemented diet low-dose streptozotocin within 3 months. Diabetic received ALA (100 mg/kg/d, orally) for 5 weeks before induction. The myocardium underwent 35 minutes ischemia through left anterior descending (LAD) coronary artery ligation 60 reperfusion. Post consisted six cycles 10/10 seconds algorithm applied early stage release creatine kinase (CK) as enzyme measured with ELISA. protein expression markers evaluated western blotting tissue scored Masson’s trichrome staining. Results: alone had no considerable cardiac marker CK, while somehow positive impact. However, application ALA-pretreated significantly reduced comparison untreated group. In addition, this combination therapy decreased Smad3 transforming growth factor-β (TGF-β) improved compared Conclusion: Combination pretreatment evoked cardio-protective improving attenuating diabetes. combined treatment can be recommended useful strategy to restore cardio-protection hearts comorbidity.