IRES-Dependent Second Gene Expression Is Significantly Lower Than Cap-Dependent First Gene Expression in a Bicistronic Vector
نویسندگان
چکیده
منابع مشابه
Translational efficiency of EMCV IRES in bicistronic vectors is dependent upon IRES sequence and gene location.
The internal ribosomal entry site (IRES)from encephalomyocarditis virus (EMCV) is a popular RNA element used widely in experimental and pharmaceutical applications to express proteins in eukaryotic cells or cell-free extracts. Inclusion of the wild-type element in monocistronic or bicistronic messenger RNAs (mRNAs) confers a high level of cap-independent translation activity to appropriately co...
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ژورنال
عنوان ژورنال: Molecular Therapy
سال: 2000
ISSN: 1525-0016
DOI: 10.1006/mthe.2000.0050