Ionic calcium content of light dense human red cells separated by percoll density gradients
نویسندگان
چکیده
منابع مشابه
Binding of immunoglobulin classes to subpopulations of human red blood cells separated by density-gradient centrifugation.
Human erythrocytes (RBC) from whole blood were separated according to their specific densities by centrifugation on a polyvinyl-pyrrolidine-coated colloidal silica matrix (Percoll) into four major subpopulations. By indirect immunofluorescence assay, the most dense RBC subpopulation, with specific density greater than 1.110 g/ml (3%-5% of total RBC), was positive for membrane-bound immunoglobul...
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Although there is evidence that the deformability of the entire red blood cell (RBC) decreases during aging, reports on changes in relevant specific properties associated with the aging process are limited and not in total agreement. The purpose of this study was to evaluate some of the factors that might contribute to this decreased deformability. Geometric, osmotic, and membrane mechanical pr...
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Frozen-thawed semen from six Boer bucks was pooled and used to compare the recently available commercial product CapriPure with Percoll for sperm quality. Sperm quality parameters such as sperm motility, concentration, membrane integrity (PI/CFDA), acrosome integrity (FITC-PNA) and mitochondrial activity (JC-1) were evaluated before and after sperm processing using CapriPure and Percoll density...
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Human thymocytes and peripheral blood lymphocytes were separated on the basis of density on Percoll gradients and the different cell populations tested regarding DNA synthesis after addition of mercuric chloride. Thymocytes with a density of 1.065-1.067 g/ml and peripheral blood lymphocytes with a density of 1.063-1.065 g/ml were maximally stimulated with mercuric chloride as well as with mitog...
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ژورنال
عنوان ژورنال: Biochimica et Biophysica Acta (BBA) - Biomembranes
سال: 1997
ISSN: 0005-2736
DOI: 10.1016/s0005-2736(96)00141-1