Ion transport in cultured pig tracheal submucosal gland acinar cells studied by X-ray microanalysis

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Ion transport in cultured pig tracheal submucosal gland acinar cells studied by X-ray microanalysis.

The submucosal glands of the airway may contribute more to the airway fluid than the surface epithelium. The cellular mechanisms underlying the regulation of electrolyte and water transport in airway submucosal glands are, however, still poorly understood. Therefore, we attempted to establish a cell culture system to facilitate study of this regulation. Acinar cells were isolated by enzymatic d...

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Multiple intracellular pathways for regulation of chloride secretion in cultured pig tracheal submucosal gland cells.

Tracheal submucosal glands are of great relative importance in the secretion of chloride and water to the airway lumen. This study aimed to examine whether the cystic fibrosis transmembrane conductance regulator (CFTR) is involved in cyclic adenosine monophosphate (cAMP) or Ca2+-activated Cl- secretion. Regulation of Cl- secretion in cell cultures derived from pig tracheal submucosal gland acin...

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Intracellular calcium store and transport of elements in acinar cells of the salivary gland determined by electron probe X-ray microanalysis.

Electron probe X-ray microanalysis using freshly frozen hydrated and dried thin sections of dog submandibular gland was performed to determine the distribution of elements and water in the acinar cells of resting and stimulating states. The results obtained are as follows: (a) The secretory granules contained high concentrations of Ca and S while high concentrations of K and P were present in t...

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Patterns of iron distribution in liver cells in beta-thalassemia studied by X-ray microanalysis.

BACKGROUND AND OBJECTIVES beta-thalassemia is an important public health problem in the countries bordering the Mediterranean sea. One of the major consequences of this disorder, primarily (due to an ineffective erythropoiesis) or secondarily to blood transfusions (which are necessary for the patient's survival), is iron storage. Applying X-ray microanalysis we wanted to demonstrate the differe...

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Chloride secretion by cultures of pig tracheal gland cells.

Malfunction of airway submucosal glands contributes to the pathology of cystic fibrosis (CF), and cell cultures of CF human airway glands show defects in Cl(-) and water transport. Recently, a transgenic pig model of CF (the CF pig) has been developed. Accordingly, we have developed cell cultures of pig airway gland epithelium for use in investigating alterations in gland function in CF. Our cu...

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ژورنال

عنوان ژورنال: European Respiratory Journal

سال: 1997

ISSN: 0000-0000,0903-1936

DOI: 10.1183/09031936.97.10102204