Invertase Immobilization on Magnetite Nanoparticles for Efficient Fructooligosaccharide Generation: A Comprehensive Kinetic Analysis and Reactor Design Strategy

This study investigated the effectiveness of immobilizing Saccharomyces cerevisiae invertase (SInv) on magnetite nanoparticles to produce fructooligosaccharides (FOSs). Based existing literature and accompanied by parameter estimation, a modified kinetic model was employed represent kinetics sucrose hydrolysis transfructosylation using SInv immobilized nanoparticle surfaces. utilized simulate performance batch reactors for both free enzymes. The maximum FOS concentration enzyme determined be 123.1 mM, while case achieved slightly higher 125.4 mM. Furthermore, continuous stirred-tank reactor (CSTR) developed enzyme, resulting in 73.96 mM at reactor’s outlet dilution rate 14.2 h−1. To examine impact glucose inhibition production, oxidase reaction mechanism integrated into fitted theoretical model. In reactor, reduction or elimination reactive media led 2.1% increase production. Immobilizing biocatalyst enhanced overall SInv. immobilization approach also holds potential coupling onto functionalized minimize inhibition, thereby improving synthesis facilitating optimal recovery reuse.