Introduction of H2-Uptake Hydrogenase Genes Into Rhizobial Strains Improves Symbiotic Nitrogen Fixation in Vicia sativa and Lotus corniculatus Forage Legumes

Biological nitrogen fixation by the Rhizobium -legume symbiosis allows conversion of atmospheric into ammonia within root nodules mediated nitrogenase enzyme. Nitrogenase activity results in evolution hydrogen as a result side reaction intrinsic to this Some rhizobia, and also other fixers, induce NiFe uptake hydrogenase (Hup) recycle produced nitrogenase, thus improving efficiency process. In work we report generation symbiotic behavior hydrogenase-positive leguminosarum Mesorhizobium loti strains effective vetch ( Vicia sativa) birsfoot trefoil Lotus corniculatus ) forage crops, respectively. The ability recycling was transferred these through incorporation hup minitransposon Tn HB100 , leading full nodules. Inoculation plants with engineered led significant increases levels incorporated host legumes. level improvement performance dependent on recipient strain legume host. These indicate that has potential improve plants.