Intranasal Nanotransferosomal Gel for Quercetin Brain Targeting: I. Optimization, Characterization, Brain Localization, and Cytotoxic Studies

Numerous neurological disorders have a pathophysiology that involves an increase in free radical production the brain. Quercetin (QER) is nutraceutical compound shields brain against oxidative stress-induced neurodegeneration. Nonetheless, its low oral bioavailability diminishes delivery. Therefore, current study aimed to formulate QER-loaded transferosomal nanovesicles (QER-TFS) situ gel for QER delivery via intranasal route. This explored impacts of lipid amount, edge activator (EA) and EA type on vesicle diameter, entrapment, cumulative amount permeated through nasal mucosa (24 h). The optimum formulation was then integrated into thermosensitive after physical morphological characteristics were assessed. Assessments optimized QER-TFS showed nanometric vesicles (171.4 ± 3.4 nm) with spherical shapes adequate entrapment efficiency (78.2 2.8%). results short-term stability high zeta potential value (−32.6 1.4 mV) confirmed their stability. Compared solution, exhibited sustained release behavior augmented permeability. CT scanning rat brains demonstrated buildup gold nanoparticles (GNPs) all treatment groups, greater level GNPs noted rats given gel. Additionally, vitro studies PCS-200-014 cells revealed minimal cytotoxicity Based these results, developed may be suitable nanocarrier targeting