Intracerebral injection of myelin basic protein (MBP) induces inflammation in brain and causes paraplegia in MBP-sensitized B6 mice

Myelin Basic Protein and a Multiple Sclerosis-related MBP-peptide Bind to Oligonucleotides

Aptamer ligands for myelin basic protein (MBP) were obtained using the systematic evolution of ligand by exponential enrichment (SELEX) method. Two clones were isolated from a pool of oligonucleotides and tested for MBP targeting. Using purified MBP, we demonstrated the binding activity of the aptamers and we also showed the affinity of MBP for oligonucleotides of specific length. Moreover, one...

Electron paramagnetic resonance spectroscopy and molecular modelling of the interaction of myelin basic protein (MBP) with calmodulin (CaM)-diversity and conformational adaptability of MBP CaM-targets.

The classic 18.5 kDa isoform of murine myelin basic protein (mMBP) has been shown to bind calmodulin (CaM) strongly and specifically in vitro. Here, we have used site-directed spin labelling (SDSL) and electron paramagnetic resonance (EPR) spectroscopy to map more precisely the sites of interaction of recombinant mMBP (rmMBP) with CaM. On the basis of these and previous experimental data, and t...

A PstI restriction fragment length polymorphism in sheep detected with a human myelin basic protein (MBP) probe.

Source and Description of Probe. The probe was obtained from a pUC8 clone, pHBP-1, that carried the complete coding sequence of the human MBP gene (Kamholz et al., 1986; American Type Culture Collection, Rockville, MD). A 1.2-kb cDNA insert was excised from pHBP-1 using the enzyme EcoRI. Method of Detection. Sheep genomic DNA was extracted from whole blood. The DNA digests were resolved in 1% a...

Nuclear Retention of MBP mRNAs in the Quaking Viable Mice

Quaking viable (qk(v)) mice fail to properly compact myelin in their central nervous systems. Although the defect in the qk(v) mice involves a mutation affecting the expression of the alternatively spliced qk gene products, their roles in myelination are unknown. We show that the QKI RNA binding proteins regulate the nuclear export of MBP mRNAs. Disruption of the QKI nucleocytoplasmic equilibri...

MBP-specific experimental autoimmune encephalomyelitis in C57BL/6 mice.