Intracellular targets for a phosphotyrosine peptidomimetic include the mitotic kinesin, MCAK
نویسندگان
چکیده
منابع مشابه
Mitotic centromere-associated kinesin (MCAK): a potential cancer drug target
The inability to faithfully segregate chromosomes in mitosis results in chromosome instability, a hallmark of solid tumors. Disruption of microtubule dynamics contributes highly to mitotic chromosome instability. The kinesin-13 family is critical in the regulation of microtubule dynamics and the best characterized member of the family, the mitotic centromere-associated kinesin (MCAK), has recen...
متن کاملC-terminus of mitotic centromere-associated kinesin (MCAK) inhibits its lattice-stimulated ATPase activity.
Mitotic centromere-associated kinesin (MCAK) is a microtubule (MT)-destabilizing molecular motor. In the present study we show that the final 8 amino acids of the C-terminus of MCAK inhibit lattice-stimulated ATPase activity of the motor. Surprisingly, loss of this C-terminal 'tail' (MCAK-Q710) leads to more rapid depolymerization of MTs relative to full-length MCAK (wt-MCAK). Biochemical and m...
متن کاملThe KinI kinesin Kif2a is required for bipolar spindle assembly through a functional relationship with MCAK
Although the microtubule-depolymerizing KinI motor Kif2a is abundantly expressed in neuronal cells, we now show it localizes to centrosomes and spindle poles during mitosis in cultured cells. RNAi-induced knockdown of Kif2a expression inhibited cell cycle progression because cells assembled monopolar spindles. Bipolar spindle assembly was restored in cells lacking Kif2a by treatments that alter...
متن کاملAurora B regulates MCAK at the mitotic centromere.
Chromosome orientation and alignment within the mitotic spindle requires the Aurora B protein kinase and the mitotic centromere-associated kinesin (MCAK). Here, we report the regulation of MCAK by Aurora B. Aurora B inhibited MCAK's microtubule depolymerizing activity in vitro, and phospho-mimic (S/E) mutants of MCAK inhibited depolymerization in vivo. Expression of either MCAK (S/E) or MCAK (S...
متن کاملMitotic Motors: Kinesin-5 Takes a Brake
A kinesin-5-dependent 'sliding filament' mechanism is commonly used to actively push apart the poles during mitotic spindle assembly and elongation, but a recent study now shows that, in C. elegans, kinesin-5 is deployed as a brake to slow down spindle-pole separation.
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ژورنال
عنوان ژورنال: Biochemical Pharmacology
سال: 2013
ISSN: 0006-2952
DOI: 10.1016/j.bcp.2013.06.024