Intracellular Mechanisms Involved in Docosahexaenoic Acid-Induced Increases in Tight Junction Permeability in Caco-2 Cell Monolayers
نویسندگان
چکیده
منابع مشابه
Peanut allergens alter intestinal barrier permeability and tight junction localisation in Caco-2 cell cultures.
BACKGROUND/AIMS Allergen absorption by epithelia may play an important role in downstream immune responses. Transport mechanisms that can bypass Peyer's patches include transcellular and paracellular transport. The capacity of an allergen to cross via these means can modulate downstream processing of the allergen by the immune system. The aim of this study was to investigate allergen-epithelial...
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We investigated the effects of 1α,25-dihydroxyvitamin D3[1,25(OH)2D3] on paracellular intestinal Ca2+absorption by determination of transepithelial electric resistance (TEER), as a measure of tight-junction ion permeability and bidirectional transepithelial45Ca2+fluxes in confluent Caco-2 cell cultures. The rise of TEER to steady-state levels of ∼2,000 Ω ⋅ cm2 was significantly attenuated by 1,...
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Chirayath, Mary V., Leszek Gajdzik, Wolfgang Hulla, Jürg Graf, Heide S. Cross, and Meinrad Peterlik. Vitamin D increases tight-junction conductance and paracellular Ca21 transport in Caco-2 cell cultures. Am. J. Physiol. 274 (Gastrointest. Liver Physiol. 37): G389–G396, 1998.—We investigated the effects of 1a,25-dihydroxyvitamin D3 [1,25(OH)2D3] on paracellular intestinal Ca21 absorption by det...
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We provide here new insights into rotavirus (RRV) pathogenicity by showing that RRV infection promotes structural and functional injuries localized at the tight junctions (TJ) in the cell-cell junctional complex of cultured polarized human intestinal Caco-2 cells forming monolayers. RRV infection resulted in a progressive increase in the paracellular permeability to [(3)H]mannitol as a function...
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Medium chain fatty acids (MCFAs) are used to enhance the permeability of mucosal tissues to hydrophilic drugs, but their mechanism of action is largely unknown. In this study, the absorption-enhancing effects of the sodium salts of two MCFAs, capric acid (C10) and lauric acid (C12), were studied in monolayers of human intestinal epithelial Caco-2 cells. Both MCFAs induced a rapid increase in ep...
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ژورنال
عنوان ژورنال: The Journal of Nutrition
سال: 2010
ISSN: 1541-6100,0022-3166
DOI: 10.3945/jn.109.120469