Intestinal Bacteria Condition Dendritic Cells to Promote IgA Production

Intestinal Bacteria Condition Dendritic Cells to Promote IgA Production

Immunoglobulin (Ig) A represents the predominant antibody isotype produced at the intestinal mucosa, where it plays an important role in limiting the penetration of commensal intestinal bacteria and opportunistic pathogens. We show in mice that Peyer's Patch-derived dendritic cells (PP-DC) exhibit a specialized phenotype allowing the promotion of IgA production by B2 cells. This phenotype inclu...

Cells Induces IgA Production by Naive IgD Human IgA-Inducing Protein from Dendritic

Generation of gut-homing IgA-secreting B cells by intestinal dendritic cells.

Normal intestinal mucosa contains abundant immunoglobulin A (IgA)-secreting cells, which are generated from B cells in gut-associated lymphoid tissues (GALT). We show that dendritic cells (DC) from GALT induce T cell-independent expression of IgA and gut-homing receptors on B cells. GALT-DC-derived retinoic acid (RA) alone conferred gut tropism but could not promote IgA secretion. However, RA p...

Fibroblast and T cells conditioned media induce maturation dendritic cell and promote T helper immune response

Dendritic cells (DCs) induce pathogen-specific T cell responses. We comprehensively studied the effects of addition of maturation stimulus, fibroblasts (fibroblast conditioned medium), PHA activated T cells (T cell conditioned medium), and mixture of fibroblast & PHA activated T cells (FCM-TCCM) conditioned media on maturation of DCs. Monocytes were cultured with GM-CSF and IL-4 for five days. ...

Ammonia production by intestinal bacteria.

Bacterial growth and the production of ammonia from urea and by deamination of peptone has been examined at various pHs in both conventional static bacterial cultures and in a continuous cultivation system. Growth occurred on primary testing of 93 out of 100 strains of aerobic Gram-negative bacteria at pH 5, and 48 out of 50 strains of Esch. coli at pH 4.6. Hydrolysis of urea by Proteus mirabil...