Internal Reference Gene Selection for Quantitative Real-Time RT-PCR Normalization in Potato Tissues
نویسندگان
چکیده
منابع مشابه
Housekeeping gene selection for real-time RT-PCR normalization in potato during biotic and abiotic stress.
Plant stress studies are more and more based on gene expression. The analysis of gene expression requires sensitive, precise, and reproducible measurements for specific mRNA sequences. Real-time RT-PCR is at present the most sensitive method for the detection of low abundance mRNA. To avoid bias, real-time RT-PCR is referred to one or several internal control genes, which should not fluctuate d...
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Today, quantitative real-time PCR is the method of choice for rapid and reliable quantification of mRNA transcription. However, for an exact comparison of mRNA transcription in different samples or tissues it is crucial to choose the appropriate reference gene. Recently glyceraldehyde 3-phosphate dehydrogenase and beta-actin have been used for that purpose. However, it has been reported that th...
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ژورنال
عنوان ژورنال: Phyton
سال: 2020
ISSN: 1851-5657
DOI: 10.32604/phyton.2020.08874