Internal amplification controls have not been employed in fungal PCR hence potential false negative results

Internal amplification controls have not been employed in fungal PCR hence potential false negative results.

Polymerase chain reaction (PCR) is subject to false negative results. Samples of fungi with the genes of interest (e.g. a disease or mycotoxin) may be categorized as negative and safe as a consequence. Fungi are eukaryotic organisms that are involved in many fields of human activity such as antibiotic, toxin and food production. Certain taxa are implicated in human, animal and plant diseases. H...

Fungal mutants not solved by internal amplification controls.

I was interested to read Rodríguez et al. (2012) on PCR to detect verrucosin producing fungi in food and cultures. However, their interpretation regarding mutated strains and internal amplification controls (IAC) appears to be incorrect, although they referred to our papers on the issue. It is worth restating to the wider scientific community that fungi may be mutated by self produced mutagens ...

Several reports have noted the occurrence of false positive results when HIV viral load assays have been used qualitatively to

False Negative Results in Clostridium difficile Testing

BACKGROUND Accurate diagnosis of Clostridium difficile infection (CDI) is paramount for patient management. The wrong diagnosis places patients at risk, delays treatment, and/ or contributes to transmission of infection in the healthcare setting. Although amplification of the toxin B gene by polymerase chain reaction (PCR) is a sensitive method for detecting toxigenic C. difficile, false negati...

False-negative culture results with fungal isolates from peritoneal dialysis fluid.

Recently, newly reported causes of fungal peritonitis associated with continuous ambulatory peritoneal dialysis were described both in this journal and in another (6, 7). These episodes threatened to go undetected due to false-negative culture results in the initial investigations. Each of these cases involved inoculation of peritoneal dialysis (PD) fluid into BACTEC (Becton Dickinson) blood cu...