Intermediates in the Bioluminescent Oxidation of Reduced Flavin Mononucleotide
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چکیده
منابع مشابه
Inhibition of the Bioluminescent Oxidation of Reduced Flavin Mononucleotide by 2-decenal.
illustrates the fact that Reactions 1 and 2 can occur in the absence of aldehyde with the production of an intermediate designated as an enzyme-sulfur peroxide. It has been shown that in the absence of aldehyde this intermediate is destroyed via a reaction indicated by 3a, in which a relatively low level light emission occurs. In the presence of aldehyde, however, the photon yield is greatly in...
متن کاملFlavin mononucleotide: the coenzyme of reduced diphosphopyridine nucleotide dehydrogenase.
From the particulate reduced nicotinamide adenine dinucleotide (DPNH) oxidase of beef heart, Mackler (3) has obtained a soluble DPNH dehydrogenase which was estimated to be approximately 90 to 95% homogenous when examined electrophoretically.l Similarly, when the enzyme was chromatographed on diethylaminoethyl cellulose with phosphate buffer, pH 7.5, in a gradient elution method (0.001 M to 0.0...
متن کاملStopped flow studies on the nonenzymatic reduction of methemoglobin by reduced flavin mononucleotide.
The nonenzymatic reduction of methemoglobin by the reduced form of flavin mononucleotide was studied under various conditions by following the reaction with a stopped flow apparatus. The reaction was very fast, compared with the reduction of the flavin by NADPH-flavin reductase of human erythrocytes, and followed a second order rate law: the rate constant (K) for the reduction of methemoglobin ...
متن کاملFlavin mononucleotide-binding flavoprotein family in the domain Archaea.
The protein (AfpA, for archaeoflavoprotein) encoded by AF1518 in the genome of Archaeoglobus fulgidus was produced in Escherichia coli and characterized. AfpA was found to be a homodimer with a native molecular mass of 43 kDa and containing two noncovalently bound flavin mononucleotides (FMNs). The cell extract of A. fulgidus catalyzed the CO-dependent reduction of AfpA that was stimulated by t...
متن کاملFlavin Mononucleotide: the Acid-extractable Flavin of the Dpnh-coenzyme Q Reductase of the Respiratory Chain.
Several D P N H dehydrogenases have been purified from heart mitochondria or heart-muscle preparationsl-L STRAUB'S enzym& has been shown by MASSEY 8 to be a form of lipoamide dehydrogenase (EC 1.6.4.3), while the relationship of the remaining preparations to the respiratory chain and to each other is obscure. The flavin extractable by acid t reatment has been reported to be FMN in some casesl, ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1963
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)68004-x