Interior of Amylopectin and Nano-Sized Amylopectin Fragments Probed by Viscometry, Dynamic Light Scattering, and Pyrene Excimer Fluorescence

Conformation of Amylose in DMSO Probed by Pyrene Excimer Fluorescence

Enzymatic synthesis of amylopectin.

The problem of branching in glycogen and starch has been the subject of interest ever since Cori and Cori (I, 2) isolated the phosphorolytic enzyme from animal tissues and Hanes (3) found a similar enzyme in plants. Examination of polysaccharides produced by these enzymes when glucose-l-phosphate was used as a substrate showed that, similar to amylose, they had a linear configuration in which t...

Probing Intramacromolecular Forces by Pyrene Excimer Fluorescence

INTRODUCTION Intramacromolecular forces (IMFs) of a macromolecule control its conformation and deformability under stress. For instance, shear will induce the elongation of a macromolecule only if it is subject to a stress that overcomes its IMFs. Since changes in the conformation of a macromolecule are usually associated with changes in the viscoelastic properties of its solution, the characte...

Internal Dynamics of Dendritic Molecules Probed by Pyrene Excimer Formation

This review exposes the current poor understanding of the internal segmental chain dynamics of dendrimers in solution probed by monitoring the process of excimer formation between pyrene labels covalently attached to the chain ends of dendrimers. The review begins by covering the bases of fluorescence and the kinetics of pyrene excimer formation before describing a procedure based on the Model ...

Fluorescence quenching of pyrene monomer and excimer by CH3I

The ̄uorescence quenching rate constants of pyrene monomer and excimer by CH3I were obtained at several temperatures in methylcyclohexane. Both quenching processes are kinetically controlled, allowing insight on the mechanism of quenching. The rate constants have both temperature-independent and temperature-dependent components. The temperature-independent component for both monomer and excimer...