Interference of Hemoglobin D in Hemoglobin A2 Measurement by Cation-Exchange HPLC

Rapid analysis of hemoglobin variants by cation-exchange HPLC.

We investigated the use of a 3.5 x 0.46 cm HPLC column packed with 5-microns particles of porous (100 nm) silica coated with polyaspartic acid for hemoglobin analysis. A 13-min gradient was produced between two mobile phases. The method is capable of separating more than 35 commonly encountered hemoglobin variants within 12 min. Hemoglobin variants identified include Bart's, acetyl F, H, A1c, F...

Cation-exchange HPLC evaluated for presumptive identification of hemoglobin variants.

A battery of relatively simple tests allows the presumptive identification of hemoglobin (Hb) variants, making unnecessary structural analysis by protein chemistry methods or DNA sequencing. The primary step in this strategy involves the use of a matrix of electrophoretic mobilities obtained under various experimental conditions. This leads to an unambiguous result in approximately 90% of the c...

Influence of hemoglobin S adducts on hemoglobin A2 quantification by HPLC.

Studies on Hemoglobin Biosynthesis: Asynchronous Synthesis of Hemoglobin a and Hemoglobin A2 by Erythrocyte Precursors.

Electrophoresis of hemoglobin on polyacrylamide gels: precise method for measurement of hemoglobin A2.

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