Interactions of plasma retinol-binding protein with its receptor. Specific binding of bovine and human retinol-binding protein to pigment epithelium cells from bovine eyes.
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چکیده
منابع مشابه
Interactions of plasma retinol-binding protein with its receptor. Specific binding of bovine and human retinol-binding protein to pigment epithelium cells from bovine eyes.
Bovine and human retinol-retinol-binding protein (RBP) were iodinated to high specific activity. At least 34% of the iodinated material was native and retained its retinol chromophore as judged by its characteristic ability to bind to plasma prealbumin. Bovine and human retinol-125I-RBP were found to bind specifically to intact isolated pigment epithelium cells from bovine eyes. The specific bi...
متن کاملBovine Retinol Pigment Epithelium
From the Medical and Research Divisions, VA Wadsworth Medical Center, and The Jules Stein Institute, UCLA School of Medicine, Los Angeles, California. Supported by USPHS grants AM 17328, EY 01190 and EY 04172 and VA research funds. *Present address: The Biological Laboratories, Harvard University, 16 Divinity Avenue, Cambridge, Massachusetts 02138. §Present address: Gastroenterology Division, D...
متن کاملRadioimmunoassay of human plasma retinol-binding protein.
A radioimmunoassay for human plasma retinol-binding protein (RBP) has been developed utilizing a double antibody precipitation technique. RBP was purified 1500- to 2000-fold by procedures described previously. A specific anti-human RBP antiserum was prepared in rabbits by three once-weekly injections of purified RBP emulsified with Freund's adjuvant. RBP was iodinated with (131)I and the RBP-(1...
متن کاملCharacterization of bovine plasma retinol-binding protein and evidence for lack of binding between it and other bovine plasma proteins.
Bovine retinol-retinol-binding protein (RBP) was isolated from serum as a free, uncomplexed protein under experimental conditions in which human, rabbit, and chicken retinol-RBP are present as tight complexes with prealbumin (thyroxine-binding protein). Purified bovine retinol-RBP formed tight complexes with purified human and chicken prealbumin in physiological ionic strength buffers as judged...
متن کاملMolecular properties of bovine interphotoreceptor retinol-binding protein.
Interphotoreceptor retinol-binding protein (IRBP) is a large retinol-carrying glycoprotein, located only in the interphotoreceptor (or subretinal) space of vertebrate eyes. It has recently been purified to apparent homogeneity. The present report presents its sedimentation, spectroscopic, and binding properties. The molecular weight of bovine IRBP, determined by sedimentation equilibrium, is 13...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1975
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)41441-5