Interactions between trypsin and its peptidic inhibitors studied by isothermal titration calorimetry (ITC)
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چکیده
منابع مشابه
Quick Start: Isothermal Titration Calorimetry (ITC)
Isothermal titration calorimetry (ITC) is a technique used to determine the thermodynamic properties of a chemical or physical equilibria. While the most abundant applications lie in the field of biochemical characterization, its use has increased in other fields because of minimal sample and buffer restrictions. The foundation of this technique is based on the simple measurement of heat, q. Be...
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The interaction of biologicalmacromolecules, whether protein-DNA, antibody-antigen, hormone-receptor, etc., illustrates the complexity and diversity of molecular recognition. The importance of such interactions in the immune response, signal transduction cascades, and gene expression cannot be overstated. It is of great interest to determine the nature of the forces that stabilize the interacti...
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LPL hydrolyzes triglycerides in plasma lipoproteins. Due to the complex regulation mechanism, it has been difficult to mimic the physiological conditions under which LPL acts in vitro. We demonstrate that isothermal titration calorimetry (ITC), using human plasma as substrate, overcomes several limitations of previously used techniques. The high sensitivity of ITC allows continuous recording of...
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Understanding the structure, folding, and interaction of membrane proteins requires experimental tools to quantify the association of transmembrane (TM) helices. Here, we introduce isothermal titration calorimetry (ITC) to measure integrin αIIbβ3 TM complex affinity, to study the consequences of helix-helix preorientation in lipid bilayers, and to examine protein-induced lipid reorganization. P...
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Cooperative binding pervades Nature. This review discusses the use of isothermal titration calorimetry (ITC) in the identification and characterisation of cooperativity in biological interactions. ITC has broad scope in the analysis of cooperativity as it determines binding stiochiometries, affinities and thermodynamic parameters, including enthalpy and entropy in a single experiment. Examples ...
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ژورنال
عنوان ژورنال: Journal of Thermal Analysis and Calorimetry
سال: 2015
ISSN: 1388-6150,1588-2926
DOI: 10.1007/s10973-015-4993-2