Interaction of the NG2 chondroitin sulfate proteoglycan with type VI collagen.

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Interaction of the NG2 chondroitin sulfate proteoglycan with type VI collagen

The NG2 chondroitin sulfate proteoglycan is a membrane-associated molecule of approximately 500 kD with a core glycoprotein of 300 kD. Both the complete proteoglycan and a smaller quantity of the 300-kD core are immunoprecipitable with polyclonal and monoclonal antibodies against purified NG2. From some cell lines, the antibodies coprecipitate NG2 and type VI collagen, the latter appearing on S...

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Inhibition of neurite growth by the NG2 chondroitin sulfate proteoglycan.

The chondroitin sulfate proteoglycans (CSPGs) have been implicated as both positive and negative modulators of axonal growth; however, the functional properties of only a few specific CSPGs have been investigated. Here we demonstrate that NG2, an integral membrane CSPG expressed on the surfaces of glial progenitor cells, inhibits neurite growth from neonatal rat cerebellar granule neurons when ...

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Interactions between chondroitin sulfate proteoglycan, fibronectin, and collagen.

A proteoglycan isolated from a rat yolk sac tumor and characterized as a chondroitin sulfate proteoglycan with a smaller amount of dermatan sulfate was studied with respect to complex formation with collagen and fibronectin. The proteoglycan co-precipitated with native collagen from neutral salt solutions at 6 degrees C and 37 degrees C. Addition of fibronectin in such precipitation mixtures re...

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Type VI collagen bound to collagen fibrils by chondroitin/dermatan sulfate glycosaminoglycan in mouse corneal stroma.

We investigated the ultrastructural localization of type VI collagen in mouse corneal stroma and its relationship to striated collagen fibrils and glycosaminoglycans, using chondroitinase ABC digestion and immunoelectron microscopy with colloidal gold particles. After chondroitinase ABC digestion, the arrangement of striated collagen fibrils was disrupted, and large spaces containing widely sca...

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Injury to the adult mammalian CNS results in reactive changes among the glial cells surrounding the site of damage. Recently, an unusual class of glial cells has been identified within the intact adult rat cerebellum on the basis of the expression of the NG2 chondroitin-sulfate proteoglycan (Levine and Card, 1987). To determine whether the cells that express the NG2 proteoglycan show reactive c...

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ژورنال

عنوان ژورنال: Journal of Cell Biology

سال: 1990

ISSN: 0021-9525,1540-8140

DOI: 10.1083/jcb.111.6.3177