Interaction of the adenovirus major core protein precursor, pVII, with the viral DNA packaging machinery
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چکیده
منابع مشابه
Interaction of the adenovirus IVa2 protein with viral packaging sequences.
We have demonstrated previously that the adenovirus L1 52/55-kDa protein binds to the viral IVa2 protein in infected cells. The significance of this interaction was unclear, however, based on the known functions of these two proteins: the 52/55-kDa protein is required for viral DNA packaging, while the IVa2 protein is a transactivator of the major late promoter (MLP). In this report, we have at...
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The DNA core of adenovirus contains two basic polypeptides V and VII. The major core polypeptide VII (M, = 18,000) is histone-like and has been shown to be derived from a larger polypeptide Pro-VII (M, = 20,000) (Anderson, C. W.. Baum, P. R., and Gesteland, R. F. (1973) J. Virol. 12, 241). Chemical studies were initiated to investigate the processing of P-VII (Pro-VII) to VII in molecular detai...
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Adenovirus type 5 (Ad5) DNA packaging is initiated in a polar fashion from the left end of the genome. The packaging process is dependent on the cis-acting packaging domain located between nucleotides 230 and 380. Seven AT-rich repeats that direct packaging have been identified within this domain. A1, A2, A5, and A6 are the most important repeats functionally and share a bipartite sequence moti...
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The adenovirus (Ad) L4-33K protein has been linked to disparate functions during infection. L4-33K is a virus-encoded alternative RNA splicing factor which activates splicing of viral late gene transcripts that contain weak 3' splice sites. Additionally, L4-33K has been indicated to play a role in adenovirus assembly. We generated and characterized an Ad5 L4-33K mutant virus to further explore ...
متن کاملAdenovirus Precursor pVII Protein Stability Is Regulated By Its Propeptide Sequence
Adenovirus encodes for the pVII protein, which interacts and modulates virus DNA structure in the infected cells. The pVII protein is synthesized as the precursor protein and undergoes proteolytic processing by viral proteinase Avp, leading to release of a propeptide sequence and accumulation of the mature VII protein. Here we elucidate the molecular functions of the propeptide sequence present...
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ژورنال
عنوان ژورنال: Virology
سال: 2005
ISSN: 0042-6822
DOI: 10.1016/j.virol.2005.01.048