Interaction of guanosine nucleotides with elongation factor 2. I. Equilibrium dialysis studies
نویسندگان
چکیده
منابع مشابه
Interaction of eukaryote elongation factor EF 1 with guanosine nucleotides and aminoacyl-tRNA.
Evidence for two species of elongation factor 1 (EF 1(A) and EF 1(B)) from calf brain has been obtained by molecular sieve chromatography on Sephadex G-150. A high molecular weight form, EF 1(A), interacts with GTP to form an EF 1(A)-GTP complex. GDP also reacts with EF 1, but unlike the reaction with GTP, an EF 1(B)-GDP complex is formed that contains a lower molecular weight and labile specie...
متن کاملElongation factor 1 from Krebs II mouse ascites cells. Interaction with guanosine nucleotides and aminoacyl-tRNA.
A procedure for the purification of elongation factor 1 (EF-1) from KrebsII mouse ascites cells is described. Ascites EF-1 occurs in multiple forms of different molecular weight like the corresponding enzymes from calf brain and rat liver. Biogel A-5m chromatography of our purest EF-1 resulted in a pattern indicating the presence of different molecular weight components. However, only a single ...
متن کاملInteraction of guanosine nucleotides with elongation factor 2. II. Effect of ribosomes and magnesium ions on guanosine diphosphate and guanosine triphosphate binding to the enzyme.
The effects of ribosomes and Mg-2plus on the binding of GDP and GTP to elongation factor 2 (EF-2) have been studied by an improved filter-binding assay. Both ribosomes and Mg-2plus strongly inhibit the binding of GDP but have apparently no effect on the GTP binding to the enzyme. An apparent stimulation by ribosomes of GTP binding to EF-2 is time-dependent and parallels a concomitant increase o...
متن کاملThe Activity of Oligonucleotides Containing Guanosine S-Triphosphate in Protein Synthesis I. THE INTERACTION OF PROTEIN SYNTHESIS ELONGATION FACTOR I WITH CYTIDYLYL (5’-3’)- GUANOSINE 5’-TRIPHOSPHATE*
The interaction of protein synthesis elongation factor 1 (EF-1) from wheat embryos and elongation factor Tu from Escherichia coli with cytidylyl(5’-3’)guanosine 5’-triphosphate(pppGpC) has been studied. The dinucleotide 5’-triphosphate interacts strongly with EF-1 as evidenced by its capacity to inhibit the binding of [3H]GTP to the factor. The analogs pGpC and GpC do not interfere with GTP bin...
متن کاملRole of guanine nucleotides in protein synthesis. Elongation factor G and guanosine 5'-triphosphate,3'-diphosphate.
The possible role of guanosine 5'-triphosphate,3'-diphosphate (pppGpp) in protein synthesis by Escherichia coli ribosomes and protein factors was examined. Although pppGpp could effectively substitute for GTP in reactions catalyzed by initiation factor 2 (ribosomal binding of fMet-tRNA and formation of N-formylmethionylpuromycin) and elongation factor T (ribosomal binding of Phe-tRNA and format...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1975
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)41955-8