منابع مشابه
Interaction of phosphorylated tryptophan hydroxylase with 14-3-3 proteins.
Rabbit brain tryptophan hydroxylase (TPH) has been expressed in insect cells (Spodoptera frugiperda) as a histidine-tagged enzyme. The specific activity of the purified fusion enzyme is 80 nmol of 5-hydroxytryptophan/min/mg. Multifunctional regulatory 14-3-3 proteins were purified from fresh bovine brain. Phosphorylation and 14-3-3 proteins play important roles in the regulation of TPH activity...
متن کاملEfficient nuclear export of p65-IκBα complexes requires 14-3-3 proteins.
There was an error published in J. Cell Sci. 119, 3695–3704. The GST-14-3-3 Coomassie stain shown in Fig. 2B was inadvertently duplicated from that shown in Fig. 2A. In Fig. 2C, the left-hand and middle GST Coomassie stains were also inadvertently duplicated, and annotation of the re-ordering of the GST-BD42 and GST-BD42A samples was omitted. Given that the data are more than 10 years old, the ...
متن کاملInteraction between Rho GTPases and 14-3-3 Proteins
The Rho GTPase family accounts for as many as 20 members. Among them, the archetypes RhoA, Rac1, and Cdc42 have been the most well-characterized. Like all members of the small GTPases superfamily, Rho proteins act as molecular switches to control cellular processes by cycling between active, GTP-bound and inactive, GDP-bound states. The 14-3-3 family proteins comprise seven isoforms. They exist...
متن کاملPhosphorylated nitrate reductase and 14-3-3 proteins. Site of interaction, effects of ions, and evidence for an amp-binding site on 14-3-3 proteins.
The inactivation of phosphorylated nitrate reductase (NR) by the binding of 14-3-3 proteins is one of a very few unambiguous biological functions for 14-3-3 proteins. We report here that serine and threonine residues at the +6 to +8 positions, relative to the known regulatory binding site involving serine-543, are important in the interaction with GF14omega, a recombinant plant 14-3-3. Also sho...
متن کاملInteraction of 14-3-3 with Signaling Proteins Is Mediated by the Recognition of Phosphoserine
The highly conserved and ubiquitously expressed 14-3-3 family of proteins bind to a variety of proteins involved in signal transduction and cell cycle regulation. The nature and specificity of 14-3-3 binding is, however, not known. Here we show that 14-3-3 is a specific phosphoserine-binding protein. Using a panel of phosphorylated peptides based on Raf-1, we have defined the 14-3-3 binding mot...
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ژورنال
عنوان ژورنال: ACS Omega
سال: 2020
ISSN: 2470-1343,2470-1343
DOI: 10.1021/acsomega.9b04413