Integrative species delimitation in the morphologically conservative Stegana subgen. Orthostegana (Diptera, Drosophilidae)

With the increasing number of cryptic species discovered in major branches subfamily Steganinae, accurate identification is becoming challenging. Possible evolutionary stasis often gives rise to formation morphological conservatism and species, as case for members Stegana (subgen. Orthostegana). In this study, boundaries all Asian Orthostegana were accessed based on two mitochondrial genes [cytochrome c oxidase subunit I ( COI ) NADH dehydrogenase 2 ND2 )] using different delimitation methods models. By integrating molecular evidence, we clearly validated independence five known identified nine new from Southwest China: (Orthostegana) aini Peng & Chen sp. nov. , S. (O.) brevivittata cuodi fuscofemorata latipalpula macrostephana mohnihei obscurala pinguitia Moreover, discussed relationship between lineage subgen. Oxyphortica, necessity taxonomic re-evaluation Neotropical with data. The high within largely outcome situ diversification adapting ecological environment.