Integrated multimodal microscopy, time-resolved fluorescence, and optical-trap rheometry: toward single molecule mechanobiology
نویسندگان
چکیده
منابع مشابه
Time-resolved fluorescence microscopy.
In fluorescence microscopy, the fluorescence emission can be characterised not only by intensity and position, but also by lifetime, polarization and wavelength. Fluorescence lifetime imaging (FLIM) can report on photophysical events that are difficult or impossible to observe by fluorescence intensity imaging, and time-resolved fluorescence anisotropy imaging (TR-FAIM) can measure the rotation...
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The fluorescence microscope, especially its confocal variant, has become a standard tool in cell biology research for delivering 3D-images of intact cells. However, the resolution of any standard optical microscope is at least 3 times poorer along the axis of the lens that in its focal plane. Here, we review principles and applications of an emerging family of fluorescence microscopes, such as ...
متن کاملTime Resolved Single Molecule Spectroscopy
A new method based on the calculation of autocorrelation functions for spectra measured at a high acquisition rate is developed to study spectral dynamics of single molecules. The technique allows for spectroscopy with time resolutions down to the luminescence lifetime. The method is used to study spectral diffusion in two-photon excitation spectra of diphenyloctatetraene molecules doped in an ...
متن کاملNanometer-localized multiple single-molecule fluorescence microscopy.
Fitting the image of a single molecule to the point spread function of an optical system greatly improves the precision with which single molecules can be located. Centroid localization with nanometer precision has been achieved when a sufficient number of photons are collected. However, if multiple single molecules reside within a diffraction-limited spot, this localization approach does not w...
متن کاملCombined optical trapping and single-molecule fluorescence
BACKGROUND Two of the mainstay techniques in single-molecule research are optical trapping and single-molecule fluorescence. Previous attempts to combine these techniques in a single experiment - and on a single macromolecule of interest - have met with little success, because the light intensity within an optical trap is more than ten orders of magnitude greater than the light emitted by a sin...
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ژورنال
عنوان ژورنال: Journal of Biomedical Optics
سال: 2007
ISSN: 1083-3668
DOI: 10.1117/1.2673245