Integrated control of axonemal dynein AAA+ motors
نویسندگان
چکیده
منابع مشابه
Axonemal dynein from Tetrahymena.
Axonemal dynein from Tetrahymena cilia can be separated on a sucrose gradient into two fractions, at least one of which appears to be polymorphic. We have been using immuno-electron microscopy in order to try and locate the different types of dynein molecules within the axonemal structure.
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Outer-arm dynein is the main engine providing the motive force in cilia. Using three-dimensional tracking microscopy, we found that contrary to previous reports Tetrahymena ciliary three-headed outer-arm dynein (αβγ) as well as proteolytically generated two-headed (βγ) and one-headed (α) subparticles showed clockwise rotation of each sliding microtubule around its longitudinal axis in microtubu...
متن کاملRegulation of Chlamydomonas flagellar dynein by an axonemal protein kinase
Genetic, biochemical, and structural data support a model in which axonemal radial spokes regulate dynein-driven microtubule sliding in Chlamydomonas flagella. However, the molecular mechanism by which dynein activity is regulated is unknown. We describe results from three different in vitro approaches to test the hypothesis that an axonemal protein kinase inhibits dynein in spoke-deficient axo...
متن کاملIdentification of the Outer-Inner Dynein Linker as a Hub Controller for Axonemal Dynein Activities
BACKGROUND In flagella, the outer dynein arm (ODA) and inner dynein arm (IDA) play distinct roles in generating beating motion. However, functional communications between the two dyneins have not been investigated. RESULTS Here, we demonstrated by cryo-electron microscopy and chemical crosslinking that intermediate chain 2 (IC2) of ODAs interacts with the dynein regulatory complex in the axon...
متن کاملModel for unidirectional movement of axonemal and cytoplasmic dynein molecules.
A model for the unidirectional movement of dynein is presented based on the structural observations and biochemical experimental results available. In this model, the binding affinity of dynein for microtubule (MT) is independent of its nucleotide state and the change between strong and weak MT-binding is determined naturally by the variation of relative orientation between the stalk and MT, as...
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ژورنال
عنوان ژورنال: Journal of Structural Biology
سال: 2012
ISSN: 1047-8477
DOI: 10.1016/j.jsb.2012.02.013