Insulin Treatment Combined with Exercise Training Does Not Prevent the Exacerbation of 12-O-Tetradecanoylphorbol-13-Acetate-Induced Inflammation in Type 2 Diabetic db/db Mice

We investigated whether 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced ear inflammation is exacerbated in type 2 diabetic db/db mice, and if it prevented by the control of blood glucose levels. Ear was assessed thickness mRNA expression pro-inflammatory cytokines IL-1beta, IL-6, TNF-alpha. Six-week-old mice received an insulin treatment combined with exercise training daily for three weeks to lower normal The other group their (db/m) saline without training. TPA-induced swelling peaked after 8 h db/m 32 mice. gene levels 24 TPA were higher than significantly suppressed HbA1c 9-week-old but did not prevent inflammatory response. These results revealed that