Insulin secretion and α-glucosidase inhibitory effects of dicaffeoylquinic acid derivatives

Abstract In this study, we investigated the effects of dicaffeoylquinic acid derivatives, including 1,4-di-O-caffeoylquinic (1,4-DCQA), 3,4-di-O-caffeoylquinic (3,4-DCQA), 3,5-di-O-caffeoylquinic (3,5-DCQA), 4,5-di-O-caffeoylquinic (4,5-DCQA), and 1,5-di-O-caffeoylquinic (1,5-DCQA) on glucose-stimulated insulin secretion (GSIS) activity α-glucosidase were compared in rat INS-1 pancreatic β-cells. The inhibitory activities derivatives as follows: 1,4-DCQA > 1,5-DCQA 3,4-DCQA 4,5-DCQA 3,5-DCQA. cells, showed no cytotoxic effect at any concentration (2.5–10 μM). addition, GSIS 3,5-DCQA 1,5-DCQA. Treatment cells with resulted a marked increase protein expression extracellular signal-regulated kinases (ERK), receptor substrate-2 (P-IRS-2), Akt, phosphoinositide 3-kinase (P-PI3K), duodenal homeobox-1 (PDX-1), which might be related to its cells. These findings indicate that location dicaffeoyl functional group influences anti-diabetic quinic acid.