Insulin-like effects of dithiothreitol on isolated rat adipocytes
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The insulin-like effects of selenate in rat adipocytes.
Selenate was found to have several insulin-like effects in rat adipocytes: stimulation of glucose transport activity by translocation of two types of glucose transporters from intracellular sites to the plasma membrane, stimulation of cAMP phosphodiesterase activity, and stimulation of ribosomal S6 protein phosphorylation. Furthermore, in intact cells addition of 1 mM selenate stimulated tyrosy...
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was plotted as a function of the percent instlin effect on uptake, control cells and cells from 24-hfasted rats gave comparable results, while cells from 48and 72-h-fasted animals still took Up less hexose for any amount of bound insulin. The effects of fasting on 3-0-methyl glucose uptake were comparable to the 2-deoxy-gltucose data. In conclusion: (a) insulin binding is increased durinig fast...
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In view of the known effect of zinc on the crystalline nature of insulin, we have investigated whether zinc alters the biologic potency of this hormone. Using the rate of lipogenesis by rat epididymal adipocytes as an index of the biologic potency of insulin, we have shown that zinc exerts a potent stimulatory effect upon lipogenesis in vitro similar to, but quite independently of, insulin and ...
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We have studied the in vitro effects of dexamethasone on isolated rat adipocytes at concentrations of dexamethasone therapeutically achieved in man. Glucose oxidation, glucose transport, and insulin binding were assessed. In dexamethasone-treated cells, glucose oxidation was decreased by 30-40% both in the absence of insulin (basal state) and at low insulin levels (less than 25 mu/ML). At maxim...
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Insulin action on glucose transport in isolated rat adipocytes was assessed using the method originally reported by Whitesell et al. Insulin increased the Vmax of glucose transport without affecting the Km. So far as concentration dependence of insulin action it had pH and temperature dependency, and analogue specificity quite similar to previous reports. The lag phase (time necessary for full ...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1981
ISSN: 0306-3283
DOI: 10.1042/bj2000425