Insight into Paphiopedilum x Dalatense Aver. (Orchidaceae) origin based on morphological and molecular markers

Paphiopedilum callosum, villosum and ´ dalatense are endangered species currently listed as Convention on International Trade in Endangered Species of Wild Fauna Flora (CITES, 2019) Appendix I species. Thus, the international trade for these naturally occurring is forbidden. However, vitro propagated materials their inter-species hybrids forced by breeders can be traded commercial purposes. P. an endemic slipper orchid Vietnam with high aesthetic value. Currently, natural reserves this have been greatly reduced overexploitation. In study, was demonstrated to reversible hybrid between callosum based both morphological molecular markers. possesses intermediate characteristics which could recorded leaf, flower its components such lip, petal, dorsal sepal. There were 23 sites ITS1-5.8S rRNA-ITS2 region sequence possess nucleotide characteristic villosum. two out three investigated samples possessed partial matK gene identical corresponding last sample callosum. Proving essentially scientific base breeding artificial hybridization contributes avoid overexploiting resources.