Insect cells respiratory activity in bioreactor
نویسندگان
چکیده
منابع مشابه
Human Papillomavirus Type16- L1 VLP Production in Insect Cells
Objective(s): Infection by high-risk papillomavirus is regarded as the major risk factor in the development of cervical cancer. Recombinant DNA technology allows expression of the L1 major capsid protein of HPV in different expression systems, which has intrinsic capacity to self-assemble into viral-like particles (VLP). VLPS are non-infectious, highly immunogenic and can elicit neutralizing...
متن کاملA re-usable wave bioreactor for protein production in insect cells
Wave-mixed bioreactors have increasingly replaced stainless steel stirred tank reactors in seed inoculum productions and mammalian cell-based process developments. Pre-sterilized, single-use plastic bags are used for cultivation, eliminating the risk of cross-contamination and cleaning procedures. However, these advantages come with high consumable costs which is the main barrier to more uptake...
متن کاملTransient transfection of insect Sf-9 cells in TubeSpin® bioreactor 50 tubes
Background Sf-9 cells, derived from Spodoptera frugiperda, are widely used for recombinant protein production using the baculovirus expression vector system (BEVS). However, this results in a productive viral infection and cell lysis. Therefore, a non-lytic, plasmid-based expression system for suspension Sf-9 cells would be a valuable alternative to the BEVS for rapid, scalable, and highyieldin...
متن کاملCloning and Expression of Simian Rotavirus Spike Protein (VP4) in Insect Cells by Baculovirus Expression System
Background: VP4 protein is as spikes on rotavirus outer capsid shell which is responsible for virus attachment to the host. VP4 induces production of neutralizing antibodies which could be used for serotyping of different isolates. Methods: Simian rotavirus SA11 gene 4 cDNA was cloned into a cloning plasmid pDONRTM by recombination reaction using clonase II enzyme mix. The resulting clone was c...
متن کاملRespiratory mutant and liquid holding recovery inhibition in yeast cells
Background: Cell ability to recover from radiation damage is of great relevance in cancer treatment. It is often believed that the inhibition of cell ability to the liquid holding recovery (LHR) may be an indicator of the overall suppression of cell ability to recover from potentially lethal radiation damage. However, the literature contains no experimental evidence whether the LHR inhibition m...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Cytotechnology
سال: 2008
ISSN: 0920-9069,1573-0778
DOI: 10.1007/s10616-007-9118-8