Inorganic pyrophosphatases: One substrate, three mechanisms

Microbial inorganic pyrophosphatases.

INTRODUCTION........................................... 169 INTRACELLULAR LOCALIZATION........................................... 169 REGULATION OF PPase SYNTHESIS........................................... 170 Effects of Culture Conditions ........................................... 170 PPase Mutants........................................... 170 Inhibition of Cell Division and Macromolecular ...

A novel subfamily of monomeric inorganic pyrophosphatases in photosynthetic eukaryotes.

Two sPPases (soluble inorganic pyrophosphatases, EC 3.6.1.1) have been isolated from the microalga Chlamydomonas reinhardtii. Both are monomeric proteins of organellar localization, the chloroplastic sPPase I [Cr (Ch. reinhardtii)-sPPase I, 30 kDa] is a major isoform and slightly larger protein than the mitochondrial sPPase II (Cr-sPPase II, 24 kDa). They are members of sPPase family I and are ...

Active site interactions in oligomeric structures of inorganic pyrophosphatases.

Recent progress in studies of the mode of action of cytoplasmic inorganic pyrophosphatases is mainly due to the analysis of a dozen and a half structures of the apoenzyme, its complexes, and mutants. However, despite considerable research on the mechanism of action of these enzymes, many important problems remain unclear. Among them is the problem of active site interactions in oligomeric struc...

Enzyme molecular choreography — Studies of soluble inorganic pyrophosphatases

............................................................................................................... 8
 1.
 INTRODUCTION.................................................................................................. 9
 1.1
 ENZYME MECHANISMS ....................................................................................... 9
 1.1.1
 Kinetic mechanism and its structural interp...

Inorganic Reaction Mechanisms