Innovative Optogenetic Strategies for Vision Restoration
نویسندگان
چکیده
منابع مشابه
A Robust Optomotor Assay for Assessing the Efficacy of Optogenetic Tools for Vision Restoration
Purpose To develop an animal behavioral assay for the quantitative assessment of the functional efficacy of optogenetic therapies. Methods A triple-knockout (TKO) mouse line, Gnat1-/-Cnga3-/-Opn4-/-, and a double-knockout mouse line, Gnat1-/-Cnga3-/-, were employed. The expression of channelrhodopsin-2 (ChR2) and its three more light-sensitive mutants, ChR2-L132C, ChR2-L132C/T159C, and ChR2-1...
متن کاملBiophysical Properties of Optogenetic Tools and Their Application for Vision Restoration Approaches
Optogenetics is the use of genetically encoded light-activated proteins to manipulate cells in a minimally invasive way using light. The most prominent example is channelrhodopsin-2 (ChR2), which allows the activation of electrically excitable cells via light-dependent depolarization. The combination of ChR2 with hyperpolarizing-light-driven ion pumps such as the Cl(-) pump halorhodopsin (NpHR)...
متن کاملStrategies for Expanding the Operational Range of Channelrhodopsin in Optogenetic Vision
Some hereditary diseases, such as retinitis pigmentosa, lead to blindness due to the death of photoreceptors, though the rest of the visual system might be only slightly affected. Optogenetics is a promising tool for restoring vision after retinal degeneration. In optogenetics, light-sensitive ion channels ("channelrhodopsins") are expressed in neurons so that the neurons can be activated by li...
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Unmet need. It is beyond dispute that human population is ageing. For the first time in history, people age 65 and over will outnumber children under age 5. This trend is emerging around the globe, and will bring several challenges for health technologies. For instance, in a few decades, the loss of health and life worldwide will be greater from chronic diseases than from infectious diseases an...
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ژورنال
عنوان ژورنال: Frontiers in Cellular Neuroscience
سال: 2018
ISSN: 1662-5102
DOI: 10.3389/fncel.2018.00316