منابع مشابه
Initiation of HIV Reverse Transcription
Reverse transcription of retroviral genomes into double stranded DNA is a key event for viral replication. The very first stage of HIV reverse transcription, the initiation step, involves viral and cellular partners that are selectively packaged into the viral particle, leading to an RNA/protein complex with very specific structural and functional features, some of which being, in the case of H...
متن کاملInitiation of HIV Reverse Transcription: Is Enzyme Flipping Required?
Liu and colleagues have recently studied dynamic changes in the orientation of HIV reverse transcriptase (RT) on its nucleic acid substrate during initiation of DNA synthesis. The authors employed a single molecule FRET assay and revealed the existence of an equilibrium between polymerase-competent and "flipped" polymerase-incompetent orientations. RT flipping correlates with enzyme pausing dur...
متن کاملInitiation of HIV-2 reverse transcription: a secondary structure model of the RNA?tRNALys3 duplex
Human immunodeficiency virus type 2 (HIV-2) reverse transcription is initiated from cellular tRNA(Lys3) partially annealed to the RNA viral genome at the primer binding site (PBS). This annealing involves interactions between two highly structured RNA molecules. In contrast to HIV-1, in which the reverse transcription initiation complex has been thoroughly studied, there is still little informa...
متن کاملInitiation of HIV-1 reverse transcription and functional role of nucleocapsid-mediated tRNA/viral genome interactions.
HIV-1 reverse transcription is initiated from a tRNA(Lys)(3) molecule annealed to the viral RNA at the primer binding site (PBS). The annealing of tRNA(Lys)(3) requires the opening of its three-dimensional structure and RNA rearrangements to form an efficient initiation complex recognized by the reverse transcriptase. This annealing is mediated by the nucleocapsid protein (NC). In this paper, w...
متن کاملStructural basis for the specificity of the initiation of HIV-1 reverse transcription.
Initiation of human immunodeficiency virus type 1 (HIV-1) reverse transcription requires specific recognition of the viral genome, tRNA3Lys, which acts as primer, and reverse transcriptase (RT). The specificity of this ternary complex is mediated by intricate interactions between HIV-1 RNA and tRNA3Lys, but remains poorly understood at the three-dimensional level. We used chemical probing to ga...
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ژورنال
عنوان ژورنال: Viruses
سال: 2010
ISSN: 1999-4915
DOI: 10.3390/v2010213