Inhibition of the interaction between fibronectin and dermatopontin by Clostridium perfringens fibronectin‐binding proteins

Fibronectin (Fn) is an approximately 450 kDa glycoprotein that consists of 12 type I, 2 II, and 15–17 III modules. Fibrillation Fn important for tissue reconstitution wound healing. We previously reported Clostridium perfringens produces several Fn-binding proteins (Fbps), two which, FbpA FbpB, bind to III1-C (a fragment derived from the carboxyl-terminal two-thirds first-type module). Dermatopontin (DPT), a 22 noncollagenous extracellular matrix protein, accelerates normal collagen fibrillation induces fibrillation. DPT interacts with Fn-type III12–14 (III12–14), leading change in conformation promoting Here, we investigated effects FbpB on binding DPT-induced Both recombinant (rFbpA) (rFbpB) significantly inhibited (rIII12–14) binding, Furthermore, it was found both rFbpA rFbpB bound coated enzyme-linked avidin–biotin complex system, whereas rIII12–14 did not either or rFbpB. In conclusion, via their interaction DPT. released lysed C. cells wounds and/or infected may prevent delay healing process, subsequently exacerbating infection.