Inhibition of formylmethionyl-leucyl-phenylalanine-stimulated neutrophil chemiluminescence by human immunoglobulin A paraproteins
نویسندگان
چکیده
منابع مشابه
Influence of antibiotics on formylmethionyl-leucyl-phenylalanine-induced leukocyte chemiluminescence.
The effect of three antimicrobial agents, penicillin G, ampicillin, and chloramphenicol, on luminol-enhanced chemiluminescence of polymorphonuclear leukocytes stimulated by the chemoattractant formylmethionyl-leucyl-phenylalanine was studied. An inhibitory effect of penicillin G and of ampicillin was demonstrated, whereas chloramphenicol gave rise to an enhancement of the chemiluminescence resp...
متن کاملPattern of formylmethionyl-leucyl-phenylalanine-induced luminol- and lucigenin-dependent chemiluminescence in human neutrophils.
The stimulation of neutrophils by formylmethionyl-leucyl-phenylalanine results in a bimodal luminol-dependent chemiluminescence pattern. We observed, however, only a single peak chemiluminescence pattern in the response to the peptide when we used lucigenin as an amplifying substance. We suggest that lucigenin, the larger molecule, (510 daltons; luminol is 177 daltons) only exerts an extracellu...
متن کاملEffect of in vitro preincubation of polymorphonuclear leukocytes on formylmethionyl-leucyl-phenylalanine-induced chemiluminescence.
When polymorphonuclear leukocytes (PMNL) and soluble or particulate matter interact, the cells produce chemiluminescence. The chemotactic peptide formylmethionyl-leucyl-phenylalanine induced a two-peak chemiluminescence response in PMNL. The response was modified, both in magnitude and in the time course of the response, when PMNL were incubated at 22 degrees C for 15 to 120 min before the addi...
متن کاملN - Formylmethionyl - Leucyl - [ 3 H ] Phenylalanine Binding , Superoxide Release , and Chemotactic Responses of Human Blood Monocytes
Human blood monocytes comprise two subpopulations: one migrates to the chemoattractant. N-formylmethionylleucyl-phenylalanine (fMet-Leu-Phe). and has saturable binding sites for this peptide; the other does not migrate and exhibits little peptide binding. To determine if expression of binding sites was a function of monocyte maturation. we depleted human subjects of blood monocytes by leukapher...
متن کاملSubcellular localization and translocation of the receptor for N-formylmethionyl-leucyl-phenylalanine in human neutrophils.
The subcellular localization of N-formylmethionyl-leucyl-phenylalanine (fMLP) receptors in human neutrophils was investigated. The fMLP receptor was detected with a high-affinity, photoactivatable, radioiodinated derivative of N-formyl-methionyl-leucyl-phenylalanyl-lysine (fMLFK). Neutrophils were disrupted by nitrogen cavitation and fractionated on Percoll density gradients. fMLP receptors wer...
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ژورنال
عنوان ژورنال: Infection and Immunity
سال: 1981
ISSN: 0019-9567,1098-5522
DOI: 10.1128/iai.34.3.864-870.1981