Inhibition of cysteine proteinases in lysosomes and whole cells

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Inhibition of cysteine proteinases in lysosomes and whole cells.

Inhibitors of cysteine proteinases have been used extensively to dissect the roles of these proteinases in cells. Surprisingly though, little work has been performed to demonstrate unequivocally that the inhibitors reach and inactivate their target proteinases in cell culture or in vivo. In the present study, the permeability of lysosomes and whole cells has been studied. Benzyloxycarbonyl (Z)-...

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NADH could not be freeze-stored at -25°C in 50 mMsodium phosphate buffer (pH range 6-8 20°C) without serious loss of the nucleotide; however, no losses were seen upon freeze storage in 50 mM-Hepes buffer (pH range 7-8, 20°C). The rate of NADH degradation was greater (approx. 2-fold) in 50 mMthan in 5 mM-sodium phosphate buffer, although NADH values at both buffer concentrations were similar (5%...

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Species variations amongst proteinases in liver lysosomes.

Cathepsin B, H, L and D activities in liver lysosomes were compared between species. Although cathepsin B and D were detected in bovine, pig, chicken and rat liver, striking species differences were evident for cathepsin H and L. Cathepsin L activity was particularly high in chicken lysosomal extracts, but could not be detected in bovine and pig extracts. Whereas there was no significant cathep...

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Cysteine proteinases and the pathogenesis of amebiasis.

Amebiasis is a major cause of morbidity and mortality throughout the tropical world. Entamoeba histolytica is now recognized as a separate species from the morphologically identical E. dispar, which cannot invade. Cysteine proteinases are a key virulence factor of E. histolytica and play a role in intestinal invasion by degrading the extracellular matrix and circumventing the host immune respon...

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Cysteine proteinases also referred to as thiol proteases play an essential role in plant growth and development but also in senescence and programmed cell death, in accumulation of storage proteins such as in seeds, but also in storage protein mobilization. Thus, they participate in both anabolic and catabolic processes. In addition, they are involved in signalling pathways and in the response ...

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ژورنال

عنوان ژورنال: Biochemical Journal

سال: 1992

ISSN: 0264-6021,1470-8728

DOI: 10.1042/bj2850495