Inhibition by α-amanitin of ribonucleic acid polymerase solubilized from rat liver nuclei
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منابع مشابه
Inhibition by alpha-amanitin of ribonucleic acid polymerase solubilized from rat liver nuclei.
1. alpha-Amanitin inhibits in vitro the RNA polymerase solubilized from isolated rat liver nuclei. 2. In contrast with previous observations with whole nuclei, the inhibition occurs approximately to the same extent in the presence and in the absence of ammonium sulphate. 3. Evidence is presented that the toxin acts by interacting with the enzyme itself and not with DNA or other components.
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1. By using ultrasonic treatment in media of high ionic strength, the RNA polymerase activities associated with prostatic nuclei and nucleoli can be completely solubilized. Such enzyme preparations are entirely dependent on the provision of added DNA for full activity. 2. The solubilized enzymes from the nucleolar and extranucleolar regions can be separated by ion-exchange chromatography. 3. Ba...
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The RNA polymerase activity present in the cytoplasm of BHK cells infected with vaccinia virus is not affected by rifampicin or by alpha-amanitin.
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merase (EC 2.7.7.6) have been demonstrated in eukaryotic cells (Roeder & Rutter, 1969; Jacob et al., 1970a; Kedinger et al., 1970; Mondal et al., 1970; Horgen & Griffin, 1971; Strain et al., 1971). RNA polymerase I (or A), which is associated with the nucleolus (Roeder & Rutter, 1970), is probably engaged in the transcription of nucleolar genes to form rRNA (Blatti et al., 1970; Tocchini-Valent...
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1. alpha-Amanitin inhibits in vitro the activity of RNA polymerase II of rat brain nuclei. 2. The toxin does not pass the blood-brain barrier, but when injected intracerebrally is highly toxic for rats, and causes inhibition of RNA polymerase II of isolated brain nuclei. 3. Intracerebral injection of alpha-amanitin 6h before training to a passive avoidance task is followed by impaired performan...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1970
ISSN: 0306-3283
DOI: 10.1042/bj1160177