Influence of Physico-Chemical Factors on the Efficiency and Metabolite Profile of Adult Pinus radiata D. Don Bud Organogenesis

Genetic improvement programs for conifer forest species face the challenge of propagating elite individuals with superior characteristics in present landscape climate change; problem is focused on fact that when these have shown desirable traits, they changed phase and therefore lost ability to be propagated by traditional methods. Based our previous works Pinus spp. regeneration adult trees through organogenesis trying improve protocol radiata, objective was analyze influence collection dates different 6-benzyladenine (BA) concentrations first shoot induction, as well effect light types success root induction. Moreover, we were interested studying abovementioned physico-chemical factors amino acid carbohydrate content shoots developed vitro. Reinvigorated obtained both BA (22 or 44 μM), although highest concentration showed best results terms induction (explants forming (46%) number per explant (1.95 ± 0.52)) using initial explants collected week February. The percentage (EFS) genotype-dependent. Explants from genotype A induced EFS (91%). With respect treatment applied, significant differences (20%) roots (4.62 0.65) observed cultured under white FL. Finally, phases rooting process detected amounts fructose, glucose sucrose threonine tyrosine.