Influence of hemoglobin S adducts on hemoglobin A2 quantification by HPLC

Influence of hemoglobin S adducts on hemoglobin A2 quantification by HPLC.

Influence of Hemoglobin S Adducts on Hemoglobin A 2 Quantification

ical goal for imprecision (CV) was 13.6% [3]. Thus the performances of all of the methods may be satisfactory. However, as Fraser and Peterson have argued, even when biological variation, not analytical variation, is the limiting source of variation in a test, there is still great advantage in using a method with the least possible imprecision and bias [4]. The aca V was the least precise instr...

Quantification of hemoglobin A2 by tandem mass spectrometry.

BACKGROUND Peptide-based analysis of whole blood using electrospray tandem mass spectrometry (MSMS) in multiple reaction monitoring (MRM) mode enables rapid detection and sequence confirmation of clinically significant hemoglobin (Hb) variants. We applied a similar, quantitative approach to the measurement of delta:beta-globin peptide ratios as potential surrogate markers of HbA(2), a biomarker...

Studies on Hemoglobin Biosynthesis: Asynchronous Synthesis of Hemoglobin a and Hemoglobin A2 by Erythrocyte Precursors.

Sparing effect of hemoglobin F and hemoglobin A2 on the polymerization of hemoglobin S at physiologic ligand saturations.

Recent interest in therapies for sickle cell anemia based on elevating fetal Hb has made accurate estimates of the sparing effect of fetal Hb (Hb F) and other non-sickle Hbs on sickle Hb (Hb S) polymerization essential. We have developed a technique, using HbCO as surrogate for HbO2, that enables us to assess the solubility of Hb S as a function of ligand saturation under conditions that mimic ...