Influence of BSA Protein on Electrochemical Response of Genosensors

The bovine serum albumin (BSA) protein is employed in genosensors as nonspecific binding blocker. In this work, the influence of BSA on electrochemical response a DNA-based biosensor explored by impedance spectroscopy (EIS) and double pulse voltammetry technique. Each buffer component hybridization studied bare gold electrodes single-stranded DNA (ssDNA)-functionalized electrodes. found to have higher sensor sensitivity. superficial modification induced greatly affects signal due steric hindrance, which decreases device Thus, we propose novel treatment method using proteinase K. K reduces improving charge transfer at electrode/solution interface enhancing genosensor proposed based rapid treatment. solution containing kept for 1 h 37 ° C obtain fragmentation then heated up 95 2 min inactivate enzyme denature fragments. was tested successfully detection Campylobacter Jejuni. Double current measurements clearly discriminate presence complementary (cDNA) fragments with any other noncomplementary (NcDNA)